hardy at mighty.fccc.edu
Tue Mar 9 09:53:00 EST 1993
In article <1993Mar7.061801.7285 at netcom.com> reason at netcom.com (Donald C.
Reason) asked for opionions about cell sorters:
>We are a relatively small group; 4 laboratories have a need for a machine
>sorts, and we envision about 3 or 4 runs a week total. We will not be
>hire a full time person to operate the instrument, although someone will
>assigned to oversee and provide support. One requirement will therefor
>of use. This task fell to me because I have had some experience with a
>laser FACS 440 and also a dual laser machine made by Coulter. Both of
>machines, and therefor my expertise, are out of date, however. My
>leanings are towards a FACS Star Plus, based on a satisfied user I have
>with and the fact that we currently have a FacsScan in house, but a
>about a new modular machine from Coulter sounded interesting. Although I
>to talk to the sales reps next week, I would like to hear from anyone who
>information and/or experience they would like to share.
We have a silimar size user group and maybe a bit more demand here with a
FACStarPlus (BD) cell sorter. For high speed sorting using surface
markers, I thing the general feeling is that this is probably the best
machine. It's definitely not the cheapest, however, nor the easiest to
maintain (although much better than that 440 - I had experience with one
of those, too). There has been some tendency in the past few years toward
air-cooled lasers which simplify the installation and are cheaper. The
EPICS ELITE is Coulter's version of this type of instrument. For cell
cycle, Ca++ flux, etc type measurements this probably is a fine solution.
BD has a new version of the Star, called the VANTAGE with some new bells
and whistles. Finally, if you can bear slow sorting (ca 100-300 cell/sec)
then there is a BD mechanical sorter that is like an upgraded FACScan for
something like $130K I believe. Wins in ease of setup, but is slow. Like
the Scan, up to 3 color.
Good luck making the decision and talk to users, not just salesbeings...
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