hamster mab - ProA ??

ryan at mbcf.stjude.org ryan at mbcf.stjude.org
Fri May 14 15:46:12 EST 1993


In article <Pine.3.05.9305130806.A6512-8100000 at post.its.mcw.edu>,
 fgarbrec at POST.ITS.MCW.EDU (Frederick Garbrecht) writes:
> Does hamster IgG bind to protein-A?  I have some hamster hybridomas
> secreting IgG, but I'm not sure if I can use ProA (or ProG) to purify.
> Thanks,
> Fred Garbrecht
> Medical College of Wisconsin
> fgarbrec at post.its.mcw.edu
> 

Hello Fred:

This was addressed by Richman et al (1982) J. Immunol. 128, 2300-2305.
They found that total hamster IgG does bind to protein A, but not quite as
efficiently as mouse IgG does. Hamster IgG binds roughly one-third as well
as does mouse IgG. No breakdown in this reference as to specific chain types.

Good luck,
Kevin.
-- 
==========================================
Kevin W. Ryan
Department of Virology & Molecular Biology
St. Jude Children's Research Hospital
Memphis, Tennessee, U.S.A.
  phone (901) 522-0411, fax (901) 523-2622
    Internet: ryan at mbcf.stjude.org
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