Methods to measure Nitric Oxide

Padmakumar Narayanan narayana at vet.vet.purdue.edu
Tue May 25 11:59:32 EST 1993


Hai there!!!
I WOULD LIKE TO GET SOME FEED-BACK REGARDING THE MEASUREMENT OF NITRIC OXIDE
PRODUCTION IN PHAGOCYTES. I KNOW THAT THERE ARE NO DIRECT METHODS TO QUANTIFY
THE PRODUCTION OF NO IN CELLS say for eg., USING FLUORESCENT DYES THAT ARE
SPECIFIC FOR NO. MOST OF THE METHODS THAT I HAVE COME ACROSS IN THE LITERATURE
ARE USING BLOCKERS AND DCFH-DA. 
ANY INFORMATION REGARDING THE USE OF ANTIBODIES TO NITRIC OXIDE SYNTHASE ARE
MOST WELCOME. I WOULD GREATLY APPRECIATE ANY HELP IN THIS REGARD.
THANK YOU,
PADMA KUMAR NARAYANAN
DEP OF PHYSIOLOGY
SVM,PURDUE UNIVERSITY
W.L. IN47907



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