Removing Con A from Con A sup

Shiv A Prasad-2 pras0005 at student.tc.umn.edu
Sun May 30 15:51:20 EST 1993


Thanks for all the suggestions.  They include:

	Running the sup over sephadex
	alpha-methyl mannoside
		add alpha-methyl-mannoside (20mM final:2ug/ml Con A
		0.1M alpha methyl D mannoside on a shaker at 37 degrees C.
	a Mannose column from Pierce

--
Shiv A. Prasad				shiv at lenti.med.umn.edu
Dept. of Microbiology			pras0005 at student.tc.umn.edu
Univ. of Minnesota



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