CTL assay: non radioactive methods

guest at imtech.ernet.in guest at imtech.ernet.in
Wed Oct 6 08:08:42 EST 1993


Hi,
I am looking for a non radioactive CTL assay. I am currently using a method
to detect Serine Esterase which is released during CTL mediated lysis. I
am facing a bit of a problem using this assay for Tcell clones and am thus
interested in finding out a method that uses a cytoplasmic marker which
will only be released from target cells on lysis. I have heard of a LDH
release assay (Lactate Dehydrogenase) can any one help me with a protocol
for this assay?

Thanking you  in advance.
Shyam
shyam at nii.ernet.in
SRF, Nationl Institute of Immunology
New Delhi India

P.S.  : Please send the reply directly to this Email address given above,
The guest account at IMtech form where this message wasposted is several
hundred KM away and can only be accessed irreat irregular intervals. Thanx
again



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