Non-adherent culture of monocytes?

[Michael Pearce]

Hmm....

I've cultured THP-1 and U937 monocyte cell-lines in teflon beakers.  The 
cells Don't stick down unless activated (by PMA for example).  However, 
once activated they can stick down hard enough to require a rubber 
policeman to get them off the teflon. I would therefore speculate that 
normal monocytes would also be able to stick down on teflon, at least 
under some conditions.

Michael Pearce