Non-adherent culture of monocytes?

HFLEIT at Path.SOM.SUNYSB.Edu HFLEIT at Path.SOM.SUNYSB.Edu
Wed Sep 29 16:46:45 EST 1993


There are two general suppliers of Teflon-PFA vials:
    Savillex Corp.
    6133 Baker Road
    Minnetonka, MN 55345
    TEL: 612-935-4100
    FAX: 612-936-2292
They make 5, 7, 17 ml capped vials for this purpose.  We've used
them.  They are reusable and autoclavable.

    Scientific Specialties Service
    P.O. Box 352
    Randallstown, MD 21133
    Tel: 301-962-0512

    They make a similar product which we've used.

    To retrieve the cultured monocyte/macrophages, we chill the
beakers on ice for about 30 min and then gently pipet the cells.  We
recover from 35 to 60% of the cells.

H.Fleit
Dept Pathology
SUNY Stony Brook




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