skonings at post.its.mcw.edu
Wed Aug 3 12:54:57 EST 1994
Dean Hewish (deanh at tigger.mel.dbe.csiro.au) wrote:
: My wife needs to distinguish active phagocytosis from passive
: absorbance in human peripheral blood macrophages. She wants to inhibit
: phagocytosis in her controls. Cytochalasin-b has been reported to be an
: inhibitor of phagocytosis but it doesn't work to 100% in her system (
: the cells still take up the particles). Does anyone know if there are
: any tricks to using cytochalasin-b and if there are any other agents
: that can be used to inhibit phagocytosis?
: Dean R. Hewish
: Cell biologist and Flow Cytometrist
: CSIRO Division of Biomolecular Engineering
: Parkville, Victoria, Australia
Why not try media free of divalent cations? They are required for binding
of particles, cell adhesion, etc. I haven't tried this myself, but have
been *warned* of this in some protocols for that reason.
Hope this helps.
Steve Konings, MS
Sr. Research Associate
Blood Research Lab
Medical College Of WI
Milwaukee - A Great Place on a Great Lake
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