My wife needs to distinguish active phagocytosis from passive
absorbance in human peripheral blood macrophages. She wants to inhibit
phagocytosis in her controls. Cytochalasin-b has been reported to be an
inhibitor of phagocytosis but it doesn't work to 100% in her system (
the cells still take up the particles). Does anyone know if there are
any tricks to using cytochalasin-b and if there are any other agents
that can be used to inhibit phagocytosis?
Dean R. Hewish
Cell biologist and Flow Cytometrist
CSIRO Division of Biomolecular Engineering
Parkville, Victoria, Australia
