lodgepa at ctrvax.vanderbilt.edu
Fri Aug 5 15:02:00 EST 1994
In article <960 at medi.rhein-main.de> Andreas Schroeder,
andi at medi.rhein-main.de writes:
>>I am working on the preparation of salivary glands mononuclear cell
>>suspension. After a 60 minutes collagenase (type II) digestion we use
>>cell strainer. However we cannot obtain a suspension clean enough to
>>gradient density separation (percoll or lympholyte M). We have
>>with cell clumping. Does anyone have a solution.
Andreas mentions the use of DNAse. I have used DNAse at 0.05% in
combination with collagenase and it works very well. Those clumps will
be gone in no time! Good luck.
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