cell suspensions

Anne Lodge lodgepa at ctrvax.vanderbilt.edu
Fri Aug 5 15:02:00 EST 1994


In article <960 at medi.rhein-main.de> Andreas Schroeder,
andi at medi.rhein-main.de writes:
>>I am working on the preparation of salivary glands mononuclear cell 
>>suspension.  After a 60 minutes collagenase (type II) digestion we use
a 
>>cell strainer.  However we cannot obtain a suspension clean enough to
use 
>>gradient density separation (percoll or lympholyte M).  We have
problems 
>>with cell clumping.  Does anyone have a solution.

Andreas mentions the use of DNAse.  I have used DNAse at 0.05% in
combination with collagenase and it works very well.  Those clumps will
be gone in no time!  Good luck.



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