Autoantibodies detection

Ctro Inmunologia Molecular monoclon%infomed at GN.APC.ORG
Sat Aug 6 18:46:27 EST 1994

   Hi Immunonetters:
   I am biochemist and I have some experience with antigen 
   quantification by mean of ELISA methods. This type of assays has a 
   good reproducibility, but assays for detection of autoantibodies are 
   very weak and in some cases the background values are more bigger 
   than the test values. I will appreciate any suggestion in this sense, 
   what kind of blocking is the best and a general schedule of some 
   particular system that somebody have been setup for this purpose.
   Thanks in advance.....
   Gilda Nunez.  
   monoclon at

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