bitter sugar

SPAGNOL at PLAUTO.CSATA.IT SPAGNOL at PLAUTO.CSATA.IT
Sat Aug 20 12:36:33 EST 1994


I cloned a glycoprotein in a cell line and have antibodies against its
sugar moiety. I do immunofluorescence and the protein is there, then I
perform an immunoblot with the same Ab and nothing stains. I arrived to load
exctracts of 10,000,000 cell on SDS PAGE- still nothing. I strongly confide
in the expertiseof  of somebody of you out there, dear colleagues.
Sincerely
Giorgio



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