W6/32 blocking of cd8+ CTL

Ress, S, Stan, Dr SRESS at uctgsh1.uct.ac.za
Fri Dec 2 10:46:15 EST 1994


>FERBAS at cyto.medsch.ucla.edu wrote about W6/32
 and blocking class 1 specific CTL


Regarding your post to bio.net, how were your clones generated
 and do they express CD56 markers or demonstrate
 LAK activity? This could be one explanation for
failure to block anti-class 1 CTL. We have had success 
with W6/32 to block CTL (Human Immunology-1985, 13, p125-
137, and 139-143. My recollection is that the dilution of 
mab was critical-surprisingly low dilutions inhibited cd8+ lines
best(1 in 200 to 1 in 800 of NH4so4 cut/ascites), but the
second paper on clones indicates that they may differ in
this regard.

I am also interested in how you generated your clones 
given the difficulty of growing CD8 clones. I would be keen to hear 
your protocol-do you use any specific cytokines aside from IL-2?

Good luck.

Regards,

Stan Ress


P.S. I will also post to the group in case there is
general interest in the discussion of this topic.

Stanley Ress
Clinical Immunology laboratory
H47 Old Main Bldg
Groote Schuur Hospital and UCT
Cape Town, 7925
South Africa

TEL:INTERN. + 021-7946176
FAX:   "    + 021-4486815
e-mail:  SRESS at uctgsh1.uct.ac.za

Stanley Ress
Clinical Immunology laboratory
H47 Old Main Bldg
Groote Schuur Hospital and UCT
Cape Town, 7925
South Africa

TEL:INTERN. + 021-7946176
FAX:   "    + 021-4486815
e-mail:  SRESS at uctgsh1.uct.ac.za



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