(second version, the first one may be walking through the cyberspace...)
I performed a human PBL culture (one hundred, actually).This culture was
performed in order to test immuno activity of a peptide called Pa. After
culturing (RPMIc, 5% FCS, 37C, %5 CO2, 24h), I measured cytokine releasing
with ELISA.
Results: IL-2, IFNg: releasing. IL-4, TNFa, IL1a, IL1b: no releasing.
I mean, this results could suggest Pa is presented and induce cytokine
releasing by Th1 Tcell. But monocytes, the only APC available into human
PBLs, appear not to be activated.
I thought APC activation (in that cytokine releasing) and presentation were
always together. But seeing my results my idea seems to be wrong.
Could anyone tell me any opinion?
Thanks.
Elena
ervazquez at mail1.cti.unav.es
