_______________________________________________________________________________TTwo
hours ago, I have writen:
>I performed a human PBL culture (one hundred, actually).This culture was
>performed in order to test immuno activity of a peptide called Pa. After
>culturing (RPMIc, 5% FCS, 37C, %5 CO2, 24h), I measured cytokine releasing
>with ELISA.
>Results: IL-2, IFNg: releasing. IL-4, TNFa, IL1a, IL1b: no releasing.
>I mean, this results could suggest Pa is presented and induce cytokine
>releasing by Th1 Tcell. But monocytes, the only APC available into human
>PBLs, appear not to be activated.
>I thought APC activation (in that cytokine releasing) and presentation were
>always together. But seeing my results my idea seems to be wrong.
>Could anyone tell me any opinion?
>Thanks.
>Elena
>ervazquez at mail1.cti.unav.es
Of course, I forgot B cells as APCs.
Anyway, I would like read some opinions....
Alan,Thank you very much.
Elena
