Synthetic Peptide Antibodies

SBehar sbehar at aol.com
Thu Dec 22 22:51:40 EST 1994


You will definitely get anti-BSA antibodies, and there is no easy way to
avoid this in the production of polyclonal antisera (although substractive
immunization is a possibility but better suited for mice and the
production of monoclonal antibodies).  However, one useful strategy is to
immunize with the peptide conjugated to a carrier such as KLH, and then
assay the antiserum using the peptide to another carrier such as BSA. 
This way, the antibodies raised against the carrier will not be detected
in the assay (for example, in an ELISA).

Sincerely, SAM BEHAR,
Samuel M. Behar, MD PhD
Seeley G. Mudd Bldg.-- Rm 508
Department of Rheumatology
Brigham and Women's Hospital
250 Longwood Avenue
Boston, MA 02115
FAX: 617-432-2799



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