Everything you say is correct. However in addtion you can make quite
good antibodies to the coupling agent. We made several dozen mabs to
peptide coupled carriers which reacted to any peptide coupled to any
carrier. so you also need to test your antibodies onBSAcouped to another
peptide (in the carrier switch given in the previous post).
On Thu, 22 Dec 1994, SBehar wrote:
> You will definitely get anti-BSA antibodies, and there is no easy way to
> avoid this in the production of polyclonal antisera (although substractive
> immunization is a possibility but better suited for mice and the
> production of monoclonal antibodies). However, one useful strategy is to
> immunize with the peptide conjugated to a carrier such as KLH, and then
> assay the antiserum using the peptide to another carrier such as BSA.
> This way, the antibodies raised against the carrier will not be detected
> in the assay (for example, in an ELISA).
>> Sincerely, SAM BEHAR,
> Samuel M. Behar, MD PhD
> Seeley G. Mudd Bldg.-- Rm 508
> Department of Rheumatology
> Brigham and Women's Hospital
> 250 Longwood Avenue
> Boston, MA 02115
> FAX: 617-432-2799
>>
