Neutrophil phagocytosis assay

Kimmy Bloom kimi at pine.circa.ufl.edu
Wed Feb 2 10:03:41 EST 1994


Padma, we are doing phagocytosis on human neutrophils using a FACscan.  We
extract them from whole blood by centrifugation over mony-poly resolving medium
and histopaque, add autologous serum after washing and lysing, and highly
fluorescent beads.  After an hour in a heated shaker-waterbath, we wash again
and run.  Whole procedure takes about 2-3 hours.  If you're interested, e-mail
me and I'll send the details.  Kimi.
In article <CKIEtr.325 at mentor.cc.purdue.edu>, narayana at vet.vet.purdue.edu
(Padmakumar Narayanan) writes:
>Hai nettors,
>Can anyone out there furnish me with a successfull protocol for neutrophil phagocytosis assay. I PREFER FLOW CYTOMETRY BASED ASSAYS.
>THANKS A TON,
>PADMA KUMAR NARAYANAN
>PURDUE CYTOMETRY LABS
>SCHOOL OF VETERINARY MEDICINE
>PURDUE UNIVERSITY
>317-494-8638; FAX-317-494-0517
>



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