Myeloma fusion partner gene sequences

choie01 at mcclb0.med.nyu.edu choie01 at mcclb0.med.nyu.edu
Thu Feb 3 01:46:24 EST 1994


Greetings to all.

I suppose that this would be the place to post this question.  I'm not
an immunologist...but

Does anyone know where I can get the cDNA sequence or GenBank
accession numbers for the light and heavy chains from the
SP2/0-Ag14(myeloma, non-secreting, mouse) and NS-1(Non-secreting
myeloma, mouse) myeloma fusion partners?

I have PCR cloned out the Vh and Vl regions with degenerate primers
from hybridomas derived from immunized Balb/c splenocytes fused with
these myeloma partners.  However, I am now uncertain whether these
sequences were derived from the myeloma fusion partner or from the
Balc/c.

Any comments or suggestions would be greatly appreciated.  Thanks in
advance for your time.  E-mail or post to this newsgroup.

Sincerely,

Elmer Choi
New York University Medical Center
Department of Pathology
email:  choie01 at mcclb0.med.nyu.edu



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