Separation of Neutrophils from Human Blood
nexejhey at rrzn-user.uni-hannover.de
Sun Feb 27 10:48:07 EST 1994
In article <2ko198$kqv at nnrp.ucs.ubc.ca>, lpss at unixg.ubc.ca (Alex Chang)
> As the title says, we are trying to separate neutrophiles from human
> peripheral blood. Currently, we are using the Ficol-Hypaque gradient
> centrifugation method. It's very time consuming. I'd to know other
> methods, more efficient ones, such as: magnetic beads, column, etc. If
> anyone know or used such methods, pleaselet me know.
You should try a discontinous PERCOLL gradient of 55%/70%.
PERCOLL is available from Sigma and Pharmacia.
100% PERCOLL = stock solution of PERCOLL plus 0.9% w/v NaCl.
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