Separation of Neutrophils from Human Blood

Thomas Wong twong at civil.ubc.ca
Mon Feb 28 03:16:47 EST 1994


I'm posting the following reply for my brother.....
________________________________________________________________________

In article <nexejhey-270294164808 at 130.75.240.46> nexejhey at rrzn-user.uni-hannover.de (Joachim Heymer) writes:
>In article <2ko198$kqv at nnrp.ucs.ubc.ca>, lpss at unixg.ubc.ca (Alex Chang)
>wrote:
>
>You should try a discontinous PERCOLL gradient of 55%/70%.
>PERCOLL is available from Sigma and Pharmacia.
>100% PERCOLL = stock solution of PERCOLL plus 0.9% w/v NaCl.
>
>JOACHIM


Has anyone tried the single step separation media from Cedarlane in
Canada and Accurate Chemical in the US? It's called Lympholyte-poly from
Cedarlane. Has anyone got it to work routinely?

Donald Wong
userDONO at mtsg.ubc.ca
Pathology, U.B.C.




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