I am wishing to use a commercial endotoxin removal gel for removal of LPS/etc. from a prep of an E.coli antigen that has ~500 EU/ml. of endotoxin acording a commercial LAL assay. I plan to use the antigen for in vitro cell proliferation and cytokine production assays in naive and primed mice. I am hoping to find hoping to obtain advice and hints as to how to make best use of the gel. I also plan to use PolymyxinB. Are there other useful techniques, please?
Appreciating your help in advance,
grad student in micro
cedar at hiv.med.umn.edu
"easy does it."