IUBio Biosequences .. Software .. Molbio soft .. Network News .. FTP

endotoxin removal from T cell antigen

John Torseth MicroBio cedar at hiv.med.umn.edu
Tue Jan 4 12:19:55 EST 1994

Dear netters:

I am wishing to use a  commercial endotoxin removal gel for removal of LPS/etc. from a prep of an E.coli antigen that has ~500 EU/ml. of endotoxin acording a   commercial LAL assay.  I plan to use the antigen for in vitro cell proliferation and cytokine production assays in naive and primed mice.  I am hoping to find  hoping to obtain advice and hints as to how to make best use of the gel.  I also plan to use PolymyxinB.  Are there other useful techniques, please?              

Appreciating your help in advance,

john torseth
grad student in micro
cedar at hiv.med.umn.edu
"easy does it."

More information about the Immuno mailing list

Send comments to us at biosci-help [At] net.bio.net