Protein A for surface IgG alignment ?

Ed Stokes ebstokes at maxwell.crd.ge.com
Thu Jan 20 17:28:15 EST 1994


Our group has been coating microtiter well plates with rabbit 
IgG in an immunoassay. Originally, we were purifying the
antiserum with protein A affinity chromatography, then
incubating the wells with purified IgG. More recently, we
have found that incubating the wells with protein A solution
first allows us to incubate with antiserum instead of
purified IgG, eliminating the tedious affinity chromatography.

The plates in this case are polystyrene.

Question: Is anyone aware of a reference which compares
(a) purified IgG adsorption to polystyrene with (b) protein A
adsorption followed by antiserum incubation? The literature
is full of references to protein A coated sepharose beads 
and gold particles, etc., but I have not been able to find 
a reference specifically related to microtiter well plate
coating...

Many thanks
Ed Stokes
ebstokes at crd.ge.com




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