In article <1994Jan18.164648.1 at earth.doane.edu> , dkatz at earth.doane.edu
> To do some of the Immuno. labs, my prep notes
>say that I need to use such things as 10 % SRBC's. When taking citrated
>from a lamb, what does this translate as in actual blood volume. I don't
>ask lightly, I have looked (maybe missed it) in Antibodies, and in
>book as well.
A 10% SRBC suspension is made by measuring the volume of packed red cells
(usually in a graduated test tube) and adding 9 parts buffer. I don't
know the hematocrit of a sheep, but it is probably between 30 and 50%,
which means that if you need 10 ml of a 10% suspension of SRBC, you would
only need 2-3.33 ml of Sheep blood. Of course you always lose some cells
during the washing, so I would start with about twice as much blood as I
Hope this helps.