E.B. STOKES WRITES:
From: ebstokes at maxwell.crd.ge.com (Ed Stokes)
Subject: Protein A for surface IgG alignment ?
Sender: usenet at crdnns.crd.ge.com (USENET News System)
Date: Thu, 20 Jan 1994 22:28:15 GMT
Our group has been coating microtiter well plates with rabbit
IgG in an immunoassay. Originally, we were purifying the
antiserum with protein A affinity chromatography, then
incubating the wells with purified IgG. More recently, we
have found that incubating the wells with protein A solution
first allows us to incubate with antiserum instead of
purified IgG, eliminating the tedious affinity chromatography.
The plates in this case are polystyrene.
Question: Is anyone aware of a reference which compares
(a) purified IgG adsorption to polystyrene with (b) protein A
adsorption followed by antiserum incubation? The literature
is full of references to protein A coated sepharose beads
and gold particles, etc., but I have not been able to find
a reference specifically related to microtiter well plate
ebstokes at crd.ge.com
I RECENTLY CAME ACROSS 2 REVIEWS PRINTED BY LABSYSTEMS RESEARCH CENTRE,
IN FINLAND. YOU CAN PROBABLY GET THEIR ADDRESS FROM THE ANNUAL
REFERENCE ISSUE PUBLISHED BY SCIENCE. THE REVIEWS ARE ENTITLED "STUDIES
ON IMMOBILIZATION OF BIOLOGICAL MATERIEALS: ADSORPTION OF ANTIBODIES TO
POLYSTYRENE" AND " STUDIES ON IMMOBILIZATION OF BIOLOGICAL MATERIALS:
ENHANCED ADSORPTION TO MODIFIED POLYSTYRENE". EACH HAS A GOOD REFERENCE
LIST AT THE END.
Distrust Simplicity. Simplify, simplify.
Alfred North Whitehead Henry David Thoreau
J. Gordon Betts
East Texas State University
Dept. of Biological Sciences
Commerce, TX 75429-3011
JB5369 at ETSUACAD.ETSU.EDU