cytotox assay

[CARMEN ELLIOT]

I am so glad someone else is having this problem with high spontaneous 
release of LDH in the Promega cytotox kit.  (Not glad that there is a 
problem, just glad that I'm not alone.)  I thought that my problem was due 
to my using primary mouse spleen cells in culture; however, if J774 macs are 
also showing high signal-to-noise ratio, it must not be the cell source.

I haven't solved the problem yet, but if I do, I will certainly pass the 
info on.  I'll keep a watch on this newsgroup for help, too.

Carmen