Some additional references on this subject:
Burleson JA, et al. J Immuno Methods 1993, 159:47-52.
Schmehl MK, et al. In Vitro Cellular & Develop Biol 1989, 25:69-81.
Bonnefoix T and Sotto J-J J Immuno methods 1994, 167:21-33.
I have used the DOS-based program at the ftp site referred to in the last
reference and it worked fine for me.
John Torseth
Grad Student in Microbiology
University of Minnesota