cell suspensions

Jean Barbeau barbeauj at AMALGAME.MEDENT.UMONTREAL.CA
Thu Jul 28 08:57:54 EST 1994


I am working on the preparation of salivary glands mononuclear cell 
suspension.  After a 60 minutes collagenase (type II) digestion we use a 
cell strainer.  However we cannot obtain a suspension clean enough to use 
gradient density separation (percoll or lympholyte M).  We have problems 
with cell clumping.  Does anyone have a solution.

thank you.


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 Jean Barbeau    E-mail: barbeauj at medent.umontreal.ca    Fax: (514) 343-2233
                  Tel: (514) 343-2366      	         Local: D-223
 
                  Faculte de medecine dentaire
                  Universite de Montreal
                  C.P. 6128, Succ. A,
                  Montreal, Quebec, Canada, H3C 3J7




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