In article <Pine.3.89.9407280930.A24016-0100000 at amalgame.MEDENT.UMontreal.CA>
barbeauj at AMALGAME.MEDENT.UMONTREAL.CA (Jean Barbeau) writes:
>I am working on the preparation of salivary glands mononuclear cell
>suspension. After a 60 minutes collagenase (type II) digestion we use a
>cell strainer. However we cannot obtain a suspension clean enough to use
>gradient density separation (percoll or lympholyte M). We have problems
>with cell clumping. Does anyone have a solution.
Cell clumping after collagenase digestion may occur due to presence of DNA
from the cell debris. DNA is a very long molecule which may clump the cells.
I never tried it, but I was told that supplementing the suspension with
DNA'se can dissolve the clumps.
--
Andreas Schroeder
