Flow cytometry and fibroblasts...

David L. Haviland, Ph.D. HAVILAND at KIDS.WUSTL.EDU
Mon Jun 13 15:02:10 EST 1994


Greetings one and all...

I need the advice of any other flow cytometry experts as well as those 
versed in immunoflourescence.  We are trying to do some studies on primary 
fibroblast cell lines.  Unfortunately we have had no success as human 
fibroblast are *hideously* autoflourescent.  A negative control being 
simply just secondary Ab that is either FITC or PE tagged gives just a good 
a histogram as what should be a positive control.  No other cell line or 
bulk cultres of cells give us this problem except for human fibroblasts.  
As far as we can tell (and my asking here a year or so ago) human 
fibroblasts are negative for Fc receptors.

Any thoughts would be appreciated!

Many thanks in advance.

David
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