Greetings one and all...
I need the advice of any other flow cytometry experts as well as those
versed in immunoflourescence. We are trying to do some studies on primary
fibroblast cell lines. Unfortunately we have had no success as human
fibroblast are *hideously* autoflourescent. A negative control being
simply just secondary Ab that is either FITC or PE tagged gives just a good
a histogram as what should be a positive control. No other cell line or
bulk cultres of cells give us this problem except for human fibroblasts.
As far as we can tell (and my asking here a year or so ago) human
fibroblasts are negative for Fc receptors.
Any thoughts would be appreciated!
Many thanks in advance.
David
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+ David L. Haviland, Ph.D. Internet:"haviland at kids.wustl.edu" +
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