Quenching FITC

Michael J. Tino mjtino at acpub.duke.edu
Wed Jun 15 16:52:34 EST 1994


I am having trouble with an assay for phagocytosis in which I would like to 
examine fluorescence of ingested FITC-labelled bacteria by macrophages.
In order to do this, I must quench the FITC outside of the macrophages to
distinguish between extra- and intracellular fluorecence.  I've
tried crystal violet and trypan blue, but with no success.  Any suggestions?

Michael Tino
michael_tino at cellbio.duke.edu

--


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                              ______
Michael James Tino            \    /   "the only measure of your words
Duke University                \  /    and your deeds  will be the love
Department of Cell Biology      \/     you leave behind when you're done."
                                         -Fred Small, "Everything Possible"
mjtino at acpub.duke.edu
michael_tino at cellbio.duke.edu

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