I am having trouble with an assay for phagocytosis in which I would like to
examine fluorescence of ingested FITC-labelled bacteria by macrophages.
In order to do this, I must quench the FITC outside of the macrophages to
distinguish between extra- and intracellular fluorecence. I've
tried crystal violet and trypan blue, but with no success. Any suggestions?
Michael Tino
michael_tino at cellbio.duke.edu
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Michael James Tino \ / "the only measure of your words
Duke University \ / and your deeds will be the love
Department of Cell Biology \/ you leave behind when you're done."
-Fred Small, "Everything Possible"
mjtino at acpub.duke.edumichael_tino at cellbio.duke.edu
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