mjtino at acpub.duke.edu (Michael J. Tino) writes:
>I am having trouble with an assay for phagocytosis in which I would like to
>examine fluorescence of ingested FITC-labelled bacteria by macrophages.
>In order to do this, I must quench the FITC outside of the macrophages to
>distinguish between extra- and intracellular fluorecence. I've
>tried crystal violet and trypan blue, but with no success. Any suggestions?
>Michael Tino
>michael_tino at cellbio.duke.edu
Some have sucessfully used anti fluorescein antibodies for this purpose.
Molecular Probes (503) 465-8300 sells antibodies for this purpose and
should be able to point you at some good references.
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| \ o IO | "If we do not find | Erik Wilson |
|-----| o GANYMEDE |anything pleasant, at | University of Illinois |
| O | o EUROPA |least we will find | (217) 359-7547 |
|----/ o CALLISTO |something new" -Voltaire| erikred at uiuc.edu |
