Quenching FITC

Erik Robert Wilson erwg0233 at uxa.cso.uiuc.edu
Thu Jun 16 07:47:00 EST 1994


mjtino at acpub.duke.edu (Michael J. Tino) writes:

>I am having trouble with an assay for phagocytosis in which I would like to 
>examine fluorescence of ingested FITC-labelled bacteria by macrophages.
>In order to do this, I must quench the FITC outside of the macrophages to
>distinguish between extra- and intracellular fluorecence.  I've
>tried crystal violet and trypan blue, but with no success.  Any suggestions?

>Michael Tino
>michael_tino at cellbio.duke.edu

Some have sucessfully used anti fluorescein antibodies for this purpose.
Molecular Probes (503) 465-8300 sells antibodies for this purpose and
should be able to point you at some good references.


-- 
|    \   o IO             | "If we do not find     | Erik Wilson              |
|-----|      o GANYMEDE   |anything pleasant, at   | University of Illinois   |
|   O |    o EUROPA       |least we will find      | (217) 359-7547           |
|----/         o CALLISTO |something new" -Voltaire| erikred at uiuc.edu         |



More information about the Immuno mailing list