RICHARD P. PHIPPS
PHIP at BPHVAX.BIOPHYSICS.ROCHESTER.EDU
Thu Jun 16 09:17:07 EST 1994
In <2tnt72$hc8 at news.duke.edu> mjtino at acpub.duke.edu writes:
> I am having trouble with an assay for phagocytosis in which I would like to
> examine fluorescence of ingested FITC-labelled bacteria by macrophages.
> In order to do this, I must quench the FITC outside of the macrophages to
> distinguish between extra- and intracellular fluorecence. I've
> tried crystal violet and trypan blue, but with no success. Any suggestions?
> Michael Tino
> michael_tino at cellbio.duke.edu
I seem to remember a protocol where the cells you wish to assay
(macrophages in this case) are labelled red (don't remember whether
they used PE or EtBr or something like that). Under a fl. microscope,
extracellular bacteria is green and phagocytosed bacteria is sort of
yellow-orange. If I remember the name of the author I will repost.
University of Rochester Cancer Center
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