house keeping standard

KONIECZNY at FRODO.MGH.HARVARD.EDU KONIECZNY at FRODO.MGH.HARVARD.EDU
Tue Jun 21 11:45:59 EST 1994


    I am trying to examine relative changes in the expression of cytokine 
genes in the antigen stimulated human cells.  This is being done by 
"direct" PCR using labeled nucleotide (not by competitive PCR).

    Does anyone have any suggestions as which house keeping gene should I 
use as a standard?  From my initial experiments I know that the expression
of actin is altered upon antigen stimulation.  So is G3PDH. 
    Does anyone have experience with beta-tubulin or pyruvate kinase 
expression under the same or similar conditions? Any other suggestions?

    Thanks in advance,

    Andrzej Konieczny 



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