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In article <2ppr1iINNf3t at medicine.wustl.edu>, HAVILAND at KIDS.WUSTL.EDU (David L. Haviland, Ph.D.) writes: There is no problem using GST fusion protein for making monoclonal antibodies. We do these routinely without cutting off the GST part. I am a new user for internet. I am sure you already have the right answer by now.