Need Help Culturing U937 cells

Linda M Ritter lritter at magnus.acs.ohio-state.edu
Fri Mar 4 10:46:36 EST 1994


To anyone who can help me:

I am a graduate student at the Ohio State University in the Molecular Genetics
department.  I have had this problem before and have just come up against it
again today.  If anyone could help me it would be greatly appreciated.

When I grow my U937 cells in RPMI with 10% FBS in flasks the grow exceptionally
well and expand quickly.  They all look healthy and the culture is uniform.
However, when I then transfer them to spinner flasks, their growth slows almost
to a stand-still and the culture does not look as uniform as it had.

I gas the flasks when I transfer the cells.  The cultures are not contaminated.

I don't know what causes this.  If anyone could help, please either e-mail me 
at lritter at magnus.ohio-state.edu or post a reply to this usernet.

Thank you for your help.

Linda



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