Serum rich medium for lymphocyte cultures- any recipes?

Kurt Schaudt olxsc01 at mailserv.zdv.uni-tuebingen.de
Fri Mar 25 10:53:49 EST 1994


Dear Netters,

We are dealing with lymphocyte (t cell) cell cultures and are using media 
prepeared with 10% serum, rest RPMI (as usual).
This media may be sufficient for short time cultures, but the question 
arises whether they REALLY are sufficiant for long time cultures too. All 
(necessary and not contained in RPMI) components are thinned tenfold, 
maybe that NORMAL (not transformed) cells suffer damage by being depraved 
from eg. hormons for a long time (and so arteficial effects are produced).
However, serum enriched media has suppressive (toxic) effects.

So, has anybody experience in prepearing serum enriched (maybe 50-70%), 
non suppressive (toxic) media?

Of course, also reports of failure are welcome!

Thank you for each reply!

Kurt Schaudt

Medical School of Tuebingen

Germany

kschaudt at mailserv.zdv.uni-tuebingen.de  



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