anti beta-gal Ab

Denise Carpenter dcarpenter at receptor.pharm.uwa.edu.au
Sun Mar 27 20:10:46 EST 1994


We ahve just purchased this antibody from Sigma of all places are are 
interested if anyone has used it to detect either endogenous or transfected 
beta gal (pSV40) in cells or tissues.  We have tried both dot blotting and 
Western blotting but have been unable to reach the sensitivity we require.  
Does anyone have any suggestions experience with using such a transfection 
assay?  Thanks in advance for any advice.

Denise Carpenter
Dept of Pharmacology
University of Western Australia
e-mail: dcarpenter at receptor.pharm.uwa.edu.au



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