Serum rich medium for lymphocyte cultures- any recipes?

William Schountz tonys at matt.ksu.ksu.edu
Sun Mar 27 23:21:33 EST 1994


I've maintained T cell clones in culture for years usining Click's
medium with 5% FBS & L-glutamine.  I use mouse T cells and restimulate
them every two weeks with peptide (no IL-2 for 3-5 days) and then in
Click's with 5U/ml rHuIL-2 to expand them.  I get my Click's from Irvine
Scientific as a powdered medium.  I've tried RPMI, but it just didn't do
the job for me.

Tony Schountz



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