I've maintained T cell clones in culture for years usining Click's
medium with 5% FBS & L-glutamine. I use mouse T cells and restimulate
them every two weeks with peptide (no IL-2 for 3-5 days) and then in
Click's with 5U/ml rHuIL-2 to expand them. I get my Click's from Irvine
Scientific as a powdered medium. I've tried RPMI, but it just didn't do
the job for me.