Help with KC Culture technique

Caro Field cm_field at postoffice.utas.edu.au
Sun Mar 27 23:18:59 EST 1994


I am currently trying to make a primary culture of keratinocytes (or
epidermal cells, generally) from murine ear and/or dorsal skin.

I have been using RPMI + 5% foetal calf serum + gentamicin (100U/ml) +
2-mercaptoethanol (0.1%) + glutamine (2mM) as a culture medium, but am
finding that cell growth is  very slow, and not satisfactory for the
purpose of my experiments.

Is there anyone who has successfully cultured keratinocytes, who would be
able to give me some advice regarding "magic ingredients" for culture
medium, or specific techniques that have been successful?

Please reply personally, rather than to this newsgroup.

Thankyou.

Carolyn Field
Department of Pathology, University of Tasmania, Australia
email: cm_field at postoffice.utas.edu.au



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