Subject: coexpression of isotypes?
From: Peter Kristensen, ptk at biobase.aau.dk
Date: Sat, 7 May 1994 14:27:37 GMT
In article <CpFs62.Ko8 at biobase.aau.dk> Peter Kristensen,
ptk at biobase.aau.dk writes:
>I have cloned Ab secreting hybridomas 15 times by limiting dilution and
>isotyped the Abs several times along the way. The result has always been
>the same : gamma 2a, gamma 3, kappa and lambda chains. After 15 clonings
>I find it difficult to believe that the cells are not monoclonal. Has
>anybody ever heard of coexpression of more than one isotype? Or do you
>think that they are still not clean?
> Best wishes Lisbeth
Lisbeth:
See the Molecular Biology/Methods-Reagents news group for my response.
>
