chemotaxis assays

elias lolis lolis at ELIRIS.MED.YALE.EDU
Thu Nov 3 20:46:32 EST 1994


Does anyone have any strong opinions on what the most effective apparatus is
for doing neutrophil chemotaxis assays?  The Boyden-chamber seems to be
heavily used but there appear to be other systems that do the job.  Specifically,
does anyone know the relative advantages/disadvantages of using the 48-well
microchamber from Neuroprobe or any of the products sold by Costar.  I am
a protein biochemist who will shortly set up to do these chemotaxis assays
(I've never done them before) and any help or references would be appreciated.

Elias




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