Your favorite assay for IL-6?

Michiaki Masuda masuda at fcs280b.ncifcrf.gov
Mon Nov 7 18:22:18 EST 1994


In article <39k6nf$qup at newsbf01.news.aol.com> jrdvmdiver at aol.com (JRDVMDIVER) writes:
>I have used the B9 bioassay for equine serum, and for supernatants.  I
>would like to have a reliable and reasonably priced ELISA to run a large
>number of samples for  equine IL-6 and TNFalpha in equine serum, CSF,
>synovial fluid, peritoneal fluid, urine, maternal fluids (ie amniotic) and
>colostrum/milk.
>Julie Robinson DVM, MVSc, MS, DABVP

   Thank you for your response. I talked with some company reps regarding
my question (i.e., availability of an ELISA kit for rat IL-6). It's truned
out that all the company that I contacted sell a kit only for human IL-6
or mouse IL-6 and their anibodies are specific enough not to cross-react
with rat IL-6. Some companies told me that they were considering
developing a kit for rat IL-6, but not yet available. Probably,
a kit for equine IL-6 won't be commercially available unless some
company perceives a sizable demand in the market.

    I seem to end up measuring IL-6 mRNA by northern analysis and
doing the bioassay if the northern results are promising.

--Michiaki
===============================================================================
Michiaki Masuda, M.D., Ph.D.
Visiting Associate
Laboratory of Molecular Oncology
National Cancer Institute
Frederick, MD 21702-1201
E-mail: Masuda at ncifcrf.gov
   



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