chemotaxis assays

Francis BEAUVAIS beauvai at world-net.sct.fr
Mon Nov 7 16:27:55 EST 1994


In message <94114105853.~INN-AGEa00163.bionet-news at dl.ac.uk>, 
lolis at ELIRIS.MED.YALE.EDU (elias lolis) wrote:
>Does anyone have any strong opinions on what the most effective apparatus is
>for doing neutrophil chemotaxis assays?  The Boyden-chamber seems to be
>heavily used but there appear to be other systems that do the job. 
>Specifically,
>does anyone know the relative advantages/disadvantages of using the 48-well
>microchamber from Neuroprobe or any of the products sold by Costar.  I am
>a protein biochemist who will shortly set up to do these chemotaxis assays
>(I've never done them before) and any help or references would be appreciated.
>
>Elias


The chemotaxis assay under agarose is a simple method for neutrophil
chemotaxis wich  is inexpensive and which can be easily  set up with
materials found  in any  lab. 

References:
J. Immunol. Meth.  (1978)  Orr W. and Ward P.A. vol. 20,  p.95-107

Methods in Enzymology  vol. 162 (Academic Press) (1988) Nelson R.D. and
Herron M.J. p.50

F. Beauvais 
beauvai at World-net.sct.fr

Francis BEAUVAIS (beauvai at World-net.sct.fr)





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