beauvai at world-net.sct.fr
Mon Nov 7 16:27:55 EST 1994
In message <94114105853.~INN-AGEa00163.bionet-news at dl.ac.uk>,
lolis at ELIRIS.MED.YALE.EDU (elias lolis) wrote:
>Does anyone have any strong opinions on what the most effective apparatus is
>for doing neutrophil chemotaxis assays? The Boyden-chamber seems to be
>heavily used but there appear to be other systems that do the job.
>does anyone know the relative advantages/disadvantages of using the 48-well
>microchamber from Neuroprobe or any of the products sold by Costar. I am
>a protein biochemist who will shortly set up to do these chemotaxis assays
>(I've never done them before) and any help or references would be appreciated.
The chemotaxis assay under agarose is a simple method for neutrophil
chemotaxis wich is inexpensive and which can be easily set up with
materials found in any lab.
J. Immunol. Meth. (1978) Orr W. and Ward P.A. vol. 20, p.95-107
Methods in Enzymology vol. 162 (Academic Press) (1988) Nelson R.D. and
Herron M.J. p.50
beauvai at World-net.sct.fr
Francis BEAUVAIS (beauvai at World-net.sct.fr)
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