Methanol as fixative for immunofluorescence

Goran Sandberg neuro gorsan at
Tue Nov 8 09:32:47 EST 1994

Has anyone an explanation to the following regular problem with thymus 
cells fixed with methanol and prepared for detection of bromodeoxyuridine 
by immunofluorescence?

The technique works fine all the time except during the summer season, 
July-Sept. This has occurred for several years now. I suspect methanol 
since another fixative in these instances gives excellent results. I have 
considered the possibility that temperature, humidity or air pressure may 
be different during the months in question, but did not find any 
correlation since we have both hot and dry days as well as cold and wet 
days during summer.

Please suggest some explanation...

Goran Sandberg                       tel: Int + 46 8 7287039
Dept Neuroscience                    fax: Int + 46 8 370757
KAROLINSKA INSTITUTET                e-mail: Goran.Sandberg at
S-171 77  Stockholm, Sweden

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