apoptosis

Rachel Caspi rcaspi at helix.nih.gov
Mon Nov 21 17:56:30 EST 1994


In article <3aj0jl$7e5 at jhunix1.hcf.jhu.edu>,
alafwalk at welchlink.welch.jhu.edu (Anne Lafond-Walker) wrote:

> Hi everyone, 
> Does anyone know of any other kit to use that locates fragmented 
> DNA in tissue sections other than ONCOR's Apoptag?  This kit works
> fine but, is expensive and the amount of reagents you receive is
> small.
> Thanks for any help
> Anne

You might want to look up TUNEL staining by Gavrieli et al. in J. Cell
Biol. 119:493 (1992). I haven't used this myself, just saw the results
displayed recently in a seminar by Jonathan Sprent here at the NIH.
Beautiful. He said that a trick is to leave the slides in the coldroom for
a couple of days with (after?) the stain. The downside: it is not a kit.
You make up the reagents. The upside: probably much cheaper than a kit.
Good luck!

-- 
Rachel R. Caspi, Ph.D.                           phone: 301-496-6409 
Laboratory of Immunology, NEI                           301-496-6394 
NIH Building 10, Room 10N222                     fax:   301-402-0485 
Bethesda, Maryland 20892                 email: rcaspi at helix.nih.gov 



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