Cloning Ab's

Let's Eat!!! hutton_rl at mricd3.apgea.army.mil
Tue Nov 29 14:39:03 EST 1994


Thanks for your reply.  The agarose trick sounds really easy; I'm going
to give it a go in the morning.  What brand of agarose do you use with this?
I've found that SeaKem Gold by FMC is the best in my hands for a combination
of low running time with good resolution, but I'm worried that your method
might not work as well with this type of agarose because of its high MP and
gel strength.  Also, what are your centrifuge parameters (g's, temp.?)

Thanx,
Robert Hutton
USArmy Institute of Chemical Defense
Edgewood, MD 



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