Commercial Source of Ab Recognizing Thioredoxin

Let's Eat!!! hutton_rl at mricd3.apgea.army.mil
Wed Nov 30 08:31:43 EST 1994


To David Kendra:

Have you heard about Invitrogen's ThioFusion(tm) Expression System?  The 
system uses a vector of theirs to attach thioredoxin to a protein (presumably 
whatever size peptide you decide to clone in).  Once this is done, the 
affinity column is relatively straightforward.  The plasmid also contains an 
enterokinase cleavage site, which will allow the thioredoxin to be released 
from the desired native protein.

I hope this helps.

Robert Hutton



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