CTL and anti-class I Abs

JAF at UNCVX1.OIT.UNC.EDU JAF at UNCVX1.OIT.UNC.EDU
Wed Nov 30 17:24:47 EST 1994


try using mhc mismatached cells as well as a variety of other mabs.  
W6/32 binds to the wrong side of class I.


On Tue, 29 Nov 1994, John Ferbas wrote:

> I have used an anti-MHC class I antibody (W632) in an attempt to demonstrate 
> MHC class I restriction in 2 CD8+ CTL clones, but have had no success.  I know 
> that the antibody is saturating the targets by FACS analysis.  Also, the 
> antibody is added before the assay and present throughout.  Is there any 
> alternative way to demonstrate class I restriction?  Your comments are greatly 
> appreciated.
> ===========================================================================================
> John Ferbas Ph.D.                                               Ferbas at cyto.medsch.ucla.edu
> UCLA MED/Cellular Immunology & Cytometry                        Voice: (310) 825 6340
> 12-236 Factor Bldg.                                             FAX:   (310) 794 2145
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