CTL and anti-class I Abs

John Ferbas ferbas at cyto.medsch.ucla.edu
Tue Nov 29 18:10:48 EST 1994


I have used an anti-MHC class I antibody (W632) in an attempt to demonstrate 
MHC class I restriction in 2 CD8+ CTL clones, but have had no success.  I know 
that the antibody is saturating the targets by FACS analysis.  Also, the 
antibody is added before the assay and present throughout.  Is there any 
alternative way to demonstrate class I restriction?  Your comments are greatly 
appreciated.
===========================================================================================
John Ferbas Ph.D.                                               Ferbas at cyto.medsch.ucla.edu
UCLA MED/Cellular Immunology & Cytometry                        Voice: (310) 825 6340
12-236 Factor Bldg.                                             FAX:   (310) 794 2145
10833 Le Conte Ave.
Los Angeles CA 90024-1745



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